Porcine Epidemic Diarrhea virus & Transmissible Gastroenteritis virus RT-qPCR Kit

Porcine Epidemic Diarrhea virus (PEDV) and Transmissible Gastroenteritis virus (TGEV) are two positive single-stranded RNA porcine coronaviruses of the genus Alphacoronavirus within the Coronaviridae family. Both TGEV and PEDV cause enteritis in pigs with very similar clinical symptoms. Major clinical signs include vomiting, watery diarrhea, dehydration, and weight loss. The mortality rate can reach greater than 90% and is inversely related to the age of pigs.

Porcine Epidemic Diarrhea virus & Transmissible Gastroenteritis virus RT-qPCR Kit is designed for the in vitro detection of PEDV and TGEV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to PEDV and TGEV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of PEDV and TGEV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. This kit clearly differentiates between these two viruses and does not detect Porcine Respiratory Coronavirus (PRCV). Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.