Salmonella spp. qPCR Kit

Salmonella is a rod-shape Gram-negative bacterium that belongs to the family of Enterobacteriaceae. Salmonella species are intracellular food-borne pathogens that cause infections due to ingestion of food contaminated by animal or human feces.

Salmonella spp. qPCR Kit is designed for the in vitro detection of Salmonella spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Salmonella spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of Salmonella spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify Salmonella spp. genomes (Kasturi K.M. et al 2001).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.