Description: ErSia13A (GH13), E.C. number 5.4.99.11, is an enzyme that produces isomaltulose (6-O-α-D-glucopyranosyl-D-fructose) and smaller amounts of trehalulose (1-O-α-D-glucopyranosyl-β-D-fructose) from sucrose from Erwinia rhapontici. Recombinant ErSia13A (GH13), purified from Escherichia coli, is a single domain family 13 Glycoside Hydrolase (GH13) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: -20 °C
Architecture: GH13
pH: 5
Temp: 30
Substrates: sucrose