Description: EcPbg4A (GH4), E.C. number 22.214.171.124, is a 6-phospho-β-glucosidase from Escherichia coli. Recombinant EcPbg4A (GH4), purified from Escherichia coli, is a single domain family 4 Glycoside Hydrolase (GH4) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: -20 °C
Temp: 37 °C
Substrates: aryl-phospho-β-glucosides, including cellobiose-6P, salicin-6P, arbutin-6P, gentiobiose-6P, methyl-b-glucoside-6P, and the chromogenic analog, p-nitrophenyl-b-D-glucopyranoside-6P