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Neopullulanase 13A, Geobacillus stearothermophilus
Neopullulanase 13A (GsNep13A), assigned the E.C. number 3.2.1.135, is a derivative of Geobacillus stearothermophilus. It is an enzyme that participates in the hydrolysis of pullulan to panose (6-α-D-glucosylmaltose). The recombinant GsNep13A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 13 (GH13) enzyme, with an N-terminal family 34 Carbohydrate Binding Module (CBM34) (see more details at www.cazy.org).
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Produced On Demand
Neopullulanase 13A (GsNep13A), assigned the E.C. number 3.2.1.135, is a derivative of Geobacillus stearothermophilus. It is an enzyme that participates in the hydrolysis of pullulan to panose (6-α-D-glucosylmaltose). The recombinant GsNep13A, purified from Escherichia coli, is a modular Glycoside Hydrolase family 13 (GH13) enzyme, with an N-terminal family 34 Carbohydrate Binding Module (CBM34) (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Glycoside Hydrolase |
| Activity | Pullulanases |
| Family | GH13 |
| EC number | 3.2.1.135 |
| Organism | Geobacillus stearothermophilus |
| Architecture | CBM34-GH13 |
| Optimal pH | 6 |
| Optimal Temperature | 55°C |
| Substrates | 1,4-α-glucans, pullulan, β-cyclodextrin (β-CD) |
Cheong et al. (2002) Biotechnol Appl Biochem. 35(Pt 1):27-34.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Glycoside Hydrolase |
| Activity | Pullulanases |
| Family | GH13 |
| EC number | 3.2.1.135 |
| Organism | Geobacillus stearothermophilus |
| Architecture | CBM34-GH13 |
| Optimal pH | 6 |
| Optimal Temperature | 55°C |
| Substrates | 1,4-α-glucans, pullulan, β-cyclodextrin (β-CD) |
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Glycoside Hydrolase |
| Activity | Pullulanases |
| Family | GH13 |
| EC number | 3.2.1.135 |
| Organism | Geobacillus stearothermophilus |
| Architecture | CBM34-GH13 |
| Optimal pH | 6 |
| Optimal Temperature | 55°C |
| Substrates | 1,4-α-glucans, pullulan, β-cyclodextrin (β-CD) |
CoA
Certificate of Analysis
