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Chondroitin AC lyase 8A, Pedobacter heparinus
Chondroitin AC lyase 8A (PhCac8A), assigned the E.C. number 4.2.2.5, is a derivative of Pedobacter heparinus. It is an enzyme that participates in the eliminative degradation of polysaccharides containing 1,4-β-D-hexosaminyl and 1,3-β-D-glucuronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groupsanhydrobond in the MurNAc residue. The recombinant PhCac8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).
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Chondroitin AC lyase 8A (PhCac8A), assigned the E.C. number 4.2.2.5, is a derivative of Pedobacter heparinus. It is an enzyme that participates in the eliminative degradation of polysaccharides containing 1,4-β-D-hexosaminyl and 1,3-β-D-glucuronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groupsanhydrobond in the MurNAc residue. The recombinant PhCac8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Polysaccharide Lyase |
| Activity | Chondroitin lyases |
| Family | PL8 |
| EC number | 4.2.2.5 |
| Organism | Pedobacter heparinus |
| Architecture | PL8 |
| Optimal pH | 6.8 |
| Optimal Temperature | 40°C |
| Substrates | chondroitin sulfate C (CS-C) |
Gu et al. (1995) Biochem J. 312 (Pt 2):569-77.
Féthière et al. (1999) J Mol Biol. 288(4):635-47.
Féthière et al. (1999) J Mol Biol. 288(4):635-47.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Polysaccharide Lyase |
| Activity | Chondroitin lyases |
| Family | PL8 |
| EC number | 4.2.2.5 |
| Organism | Pedobacter heparinus |
| Architecture | PL8 |
| Optimal pH | 6.8 |
| Optimal Temperature | 40°C |
| Substrates | chondroitin sulfate C (CS-C) |
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Polysaccharide Lyase |
| Activity | Chondroitin lyases |
| Family | PL8 |
| EC number | 4.2.2.5 |
| Organism | Pedobacter heparinus |
| Architecture | PL8 |
| Optimal pH | 6.8 |
| Optimal Temperature | 40°C |
| Substrates | chondroitin sulfate C (CS-C) |
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Certificate of Analysis
