Description: BtHep12B (PL12), E.C. number 188.8.131.52, is an enzyme that acts on linkages between acetyl-glucosamine and uronate of heparan sulfate from Bacteroides thetaiotaomicron. Recombinant BtHep12B (PL12), purified from Escherichia coli, is a single domain family 12 Pectate Lyase (PL12) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: – 20 °C
Temp: 25-37 °C
Substrates: low or unsulfated regions of heparan sulfate