Description: In molecular biology, TBE and TAE buffers are used for agarose and polyacrylamide gel electrophoresis. TBE (Tris-Borate-Acetate) buffer is suitable when analysing DNA fragments from PCR amplification, DNA isolation protocols, or DNA cloning experiments. It is adapted for separating smaller DNA fragments (less than 1500 bp on a 0.8% agarose gel). TBE has a greater buffering capacity and will give sharper resolution than TAE. However, TBE gels in general afford a poor recovery of nucleic acids compared with TAE gels.
Applications:
– Nucleic acid electrophoresis running buffer for agarose and polyacrylamide gels
Directions for use: Dilute 100 mL of TBE Buffer, 10x stock solution into 900 mL deionised water to make 1 litre of TBE Buffer. Final concentration is 89 mM Tris base, 89 mM Borate and 2 mM Na2EDTA. On dilution to 1X the user should check pH and adjust as required.
Specifications:
– Tris 0.9 M 108 g/L
– Boric acid 0.9 M 55 g/L
– Na2EDTA 0.02 M 43.5 g/L
Storage: Store at room temperature. Keep away from light.
