Description: Gentamicin sulfate is a broad-spectrum antibiotic, used as a selection agent (gentamicin-resistance gene) in cell culture and molecular biology applications. Gentamicin causes codon misreading by binding to the 30S ribosomal subunit, blocking the translocation of peptidyl-tRNA from the acceptor site to the donor site. The bactericidal effect of gentamicin on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface. Presented in approximately 640 µ/mg.
Chemical formula: C60H125N15O25S
CAS number: 1405-41-0
Soluble in: water
Storage: dry at room temperature; to increase shelf-life, store at 0-5 ºC