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SKU
MB00901
Klenow Fragment of DNA Polymerase I
Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5′ → 3′ exonuclease activity while retaining the 3′ → 5′ exonuclease activity (proofreading).
Availability
Ready to Ship
Dry Ice
Klenow Fragment of DNA Polymerase I is a truncated fragment of E. coli DNA polymerase I that lacks the 5' → 3' exonuclease activity while retaining the 3' → 5' exonuclease activity (proofreading activity). The enzyme is well suited for use in 3´-end labelling of DNA fragments for sequence analysis, for the creation of bluntends by the filling-in of 5´-overhangs or by the removal of 3´-overhangs, for second-strand synthesis of cDNA and for random primer labelling of DNA fragments.
Applications:
– 3´-end labelling of DNA fragments for sequence analysis
– Conversion of 5´-overhangs to create blunt-ends for cloning
– Removal of 3´-overhangs
– Second-strand synthesis of cDNA
– Random primer labelling of DNA fragments
Applications:
– 3´-end labelling of DNA fragments for sequence analysis
– Conversion of 5´-overhangs to create blunt-ends for cloning
– Removal of 3´-overhangs
– Second-strand synthesis of cDNA
– Random primer labelling of DNA fragments
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase |
| Product Category | Polymerases |
| Reaction Temperature | 37 °C |
| Heat Inactivation | 75 °C |
| Concentration | 5 U/μL |
– Klenow Fragment of DNA Polymerase I (300 U)
– Reaction buffer (10x)
– Reaction buffer (10x)
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase |
| Product Category | Polymerases |
| Reaction Temperature | 37 °C |
| Heat Inactivation | 75 °C |
| Concentration | 5 U/μL |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | 3' → 5' exonuclease, 5' → 3' DNA polymerase |
| Product Category | Polymerases |
| Reaction Temperature | 37 °C |
| Heat Inactivation | 75 °C |
| Concentration | 5 U/μL |
CoA
Certificate of Analysis
