Lyo NZY Reverse Transcriptase (MB40901)


100000 U


Description: Lyo NZY Reverse Transcriptase is a freeze-dried enzyme provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. Lyo NZY Reverse Transcriptase is a modified recombinant form of the Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase purified from Escherichia coli. Lyo NZY Reverse Transcriptase synthesizes the complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template in a wide range of temperatures (37-50°C). High reaction temperatures are beneficial to improve specificity and to allow synthesis of cDNA from complex templates, including those with high GC-content or with high degree of secondary structure. The enzyme lacks 3´→5´ exonuclease activity and has no RNase H activity, enabling improved synthesis of full-length cDNA even for long mRNA, using random priming. Thus, Lyo NZY Reverse Transcriptase gives high yields of cDNA up to 7 kb.

– Eco-friendly room temperature shipment
– Synthesizes of cDNA from RNA or ssDNA in only 30 min
– High yields of first-strand cDNA
– No intrinsic RNase H activity
– Thermostable – working temperature range 37-50 ºC

– First-strand cDNA synthesis for RT-PCR and RT-qPCR
– Reverse transcription at elevated temperatures (ideal for high GC-content templates)
– Synthesis of cDNA for cloning and expression
– Analysis of RNA by primer extension
– DNA labeling


Product length: up to 7 kb
Sensitivity: high
Optimal reaction temperature:  50 ºC
Speed: 30 min
Denaturing conditions: Lyo NZY Reverse Transcriptase is inhibited in the presence of metal chelators (e.g. EDTA), inorganic phosphate, pyrophosphate and polyamines. The enzyme is inactivated at 85 °C for 5 min
Liquid format available: NZY Reverse Transcriptase  (MB124)
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped with dry ice to room temperature


– Lyo NZY Reverse Transcriptase (100000 U)
– Lyo NZY-RT resuspension buffer
– Reaction Buffer (10x)
– 0.1 M DTT

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis ZN031
Certificate of Analysis NM072

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