Lyo NZY Reverse Transcriptase (MB40901)

Description: Lyo NZY Reverse Transcriptase is a freeze-dried enzyme provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. Lyo NZY Reverse Transcriptase is a modified recombinant form of the Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase purified from Escherichia coli. Lyo NZY Reverse Transcriptase synthesizes the complementary DNA strand in the presence of a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template in a wide range of temperatures (37-50°C). High reaction temperatures are beneficial to improve specificity and to allow synthesis of cDNA from complex templates, including those with high GC-content or with high degree of secondary structure. The enzyme lacks 3´→5´ exonuclease activity and has no RNase H activity, enabling improved synthesis of full-length cDNA even for long mRNA, using random priming. Thus, Lyo NZY Reverse Transcriptase gives high yields of cDNA up to 7 kb.

Features:
– Eco-friendly room temperature shipment
– Synthesizes of cDNA from RNA or ssDNA in only 30 min
– High yields of first-strand cDNA
– No intrinsic RNase H activity
– Thermostable – working temperature range 37-50 ºC

Applications: 
– First-strand cDNA synthesis for RT-PCR and RT-qPCR
– Reverse transcription at elevated temperatures (ideal for high GC-content templates)
– Synthesis of cDNA for cloning and expression
– Analysis of RNA by primer extension
– DNA labeling

Specifications:

Components:
– Lyo NZY Reverse Transcriptase (100000 U)
– Lyo NZY-RT resuspension buffer
– Reaction Buffer (10x)
– 0.1 M DTT