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β-Acetylglucosaminidase 18B, Elizabethkingia meningoseptica
β-Acetylglucosaminidase 18B (EmAcp18B), assigned the E.C. number 3.2.1.96, is a derivative of Elizabethkingia meningoseptica. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant EmAcp18B, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 18 (GH18) enzyme (see more details at www.cazy.org).
β-Acetylglucosaminidase 18B (EmAcp18B), assigned the E.C. number 3.2.1.96, is a derivative of Elizabethkingia meningoseptica. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant EmAcp18B, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 18 (GH18) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT | Storage Conditions | -30°C to -15°C | Class | Glycoside Hydrolase | Activity | Acetylglucosaminidases | Family | GH18 | EC number | 3.2.1.96 | Organism | Elizabethkingia meningoseptica | Architecture | GH18 | Optimal pH | 7.5 | Optimal Temperature | 35°C | Substrates | glycoproteins |
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Van Roey et al. (1994) Biochemistry. 33(47):13989-96.
Tarentino et al. (1992) J Biol Chem. 267(6):3868-72.
Tarentino et al. (1992) J Biol Chem. 267(6):3868-72.
Shipping Conditions | Shipping: Dry Ice to RT | Storage Conditions | -30°C to -15°C | Class | Glycoside Hydrolase | Activity | Acetylglucosaminidases | Family | GH18 | EC number | 3.2.1.96 | Organism | Elizabethkingia meningoseptica | Architecture | GH18 | Optimal pH | 7.5 | Optimal Temperature | 35°C | Substrates | glycoproteins |
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Shipping Conditions | Shipping: Dry Ice to RT | Storage Conditions | -30°C to -15°C | Class | Glycoside Hydrolase | Activity | Acetylglucosaminidases | Family | GH18 | EC number | 3.2.1.96 | Organism | Elizabethkingia meningoseptica | Architecture | GH18 | Optimal pH | 7.5 | Optimal Temperature | 35°C | Substrates | glycoproteins |
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CoA
Certificate of Analysis