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β-Acetylglucosaminidase 85A, Streptococcus pneumoniae
β-Acetylglucosaminidase 85A (SpAcp85A), assigned the E.C. number 3.2.1.96, is a derivative of Streptococcus pneumoniae. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant SpAcp85A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 85 (GH85) enzyme (see more details at www.cazy.org).
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β-Acetylglucosaminidase 85A (SpAcp85A), assigned the E.C. number 3.2.1.96, is a derivative of Streptococcus pneumoniae. It is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the (Man(GlcNAc)(2))Asn-structure: one N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact. The recombinant SpAcp85A, purified from Escherichia coli, is a single-domain Glycoside Hydrolase family 85 (GH85) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
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Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | Acetylglucosaminidases |
Family | GH85 |
EC number | 3.2.1.96 |
Organism | Streptococcus pneumoniae |
Architecture | GH85 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (3F4NP-GlcNAc), chitobiose core of N-glycans |
Abbott et al. (2009) J Biol Chem. 284(17):11676-89.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | Acetylglucosaminidases |
Family | GH85 |
EC number | 3.2.1.96 |
Organism | Streptococcus pneumoniae |
Architecture | GH85 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (3F4NP-GlcNAc), chitobiose core of N-glycans |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Glycoside Hydrolase |
Activity | Acetylglucosaminidases |
Family | GH85 |
EC number | 3.2.1.96 |
Organism | Streptococcus pneumoniae |
Architecture | GH85 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | 3-fluoro-4-nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside (3F4NP-GlcNAc), chitobiose core of N-glycans |
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