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Hyaluronate lyase 8A, Streptococcus pneumoniae
Hyaluronate lyase 8A (SpHys8A), assigned the E.C. number 4.2.2.1, is a derivative of Streptococcus pneumoniae. It is an enzyme that cleaves hyaluronate chains at a β-D-GlcNAc-1,4-β-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine. The recombinant SpHys8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).
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Produced On Demand
Hyaluronate lyase 8A (SpHys8A), assigned the E.C. number 4.2.2.1, is a derivative of Streptococcus pneumoniae. It is an enzyme that cleaves hyaluronate chains at a β-D-GlcNAc-1,4-β-D-GlcA bond, ultimately breaking the polysaccharide down to 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine. The recombinant SpHys8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
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Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Hyaluronate lyases |
Family | PL8 |
EC number | 4.2.2.1 |
Organism | Streptococcus pneumoniae |
Architecture | PL8 |
Optimal pH | 6 |
Optimal Temperature | 30°C |
Substrates | hyaluran |
Rigden and Jedrzejas. (2003) J Biol Chem. 278(50):50596-606.
Rigden et al. (2006) J Mol Biol. 358(4):1165-78.
Rigden et al. (2006) J Mol Biol. 358(4):1165-78.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Hyaluronate lyases |
Family | PL8 |
EC number | 4.2.2.1 |
Organism | Streptococcus pneumoniae |
Architecture | PL8 |
Optimal pH | 6 |
Optimal Temperature | 30°C |
Substrates | hyaluran |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Hyaluronate lyases |
Family | PL8 |
EC number | 4.2.2.1 |
Organism | Streptococcus pneumoniae |
Architecture | PL8 |
Optimal pH | 6 |
Optimal Temperature | 30°C |
Substrates | hyaluran |
CoA
Certificate of Analysis