DNA Ligase E. coli

Storage Conditions:

-85 °C to -15 °C

DNA Ligase (E. coli) is a typical DNA ligase that catalyses the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA.

Availability

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Dry Ice

Details

DNA Ligase (E. coli) is a typical DNA ligase that catalyses the formation of a phosphodiester bond between the 5´-phosphate and the 3´-hydroxyl of two adjacent DNA strands in duplex DNA. Thus, the enzyme is particularly effective to repair nicks in dsDNA although it can be used to ligate two DNA fragments with compatible cohesive ends. DNA Ligase (E. coli) is not significantly active on blunt-ended substrates. DNA Ligase (E. coli) uses NAD as a cofactor and can be heat-inactivated. The enzyme is active at a range of temperatures (4 °C - 37 °C).
Applications:
– Gene cloning
– Second-strand cDNA synthesis
– Phosphodiester bond ligations

More Information
Shipping Conditions	Dry Ice
Storage Conditions	-85 °C to -15 °C
Activity	Sticky-end ligation
Product Category	Ligases
Reaction Temperature	16 °C
Heat Inactivation	65 °C
Concentration	10 U/μL

Components

– DNA Ligase (E. coli) (10 U/μL)
– Reaction buffer (10x)

More Information
Shipping Conditions	Dry Ice
Storage Conditions	-85 °C to -15 °C
Activity	Sticky-end ligation
Product Category	Ligases
Reaction Temperature	16 °C
Heat Inactivation	65 °C
Concentration	10 U/μL

More Information
Shipping Conditions	Dry Ice
Storage Conditions	-85 °C to -15 °C
Activity	Sticky-end ligation
Product Category	Ligases
Reaction Temperature	16 °C
Heat Inactivation	65 °C
Concentration	10 U/μL

MSDS

Material Safety Data Sheets

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CoA

Certificate of Analysis

Manuals

Instruction for Use - EN

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