Supreme NZYProof DNA polymerase

Supreme NZYProof DNA polymerase is an engineered highly accurate, fast and sensitive variant of NZYProof DNA polymerase displaying a hot-start like PCR capacity. This feature is achieved by a novel hot-start technology, which inhibits both polymerase and 3’→5’ exonuclease activities and thus avoids extension of non-specifically annealed primers or primer-dimers, as well as the degradation of primers and template DNA during PCR reaction setup. Thus, Supreme NZYProof DNA polymerase generates higher specificity, sensitivity and yield during the accurate amplification of DNA. Supreme NZYProof DNA polymerase was also engineered for higher processivity, thus allowing fast PCR reactions of longer PCR products. This highly robust version of NZYProof DNA polymerase is a broad range enzyme suitable for a variety of applications, including amplification of longer (≤10 kb) and difficult PCR products, as well as site-directed mutagenesis. Supreme NZYProof DNA polymerase possesses 3’→5’ exonuclease proofreading activity that enables the polymerase to amplify DNA with increased accuracy. The error rate of Supreme NZYProof DNA polymerase is similar to that of Pfu and Kod DNA polymerases and significantly lower than the error rate of Taq DNA polymerases. It generates blunt-ended PCR products that are suitable for cloning with NZYtech’s NZY-blunt PCR cloning kit (MB121).