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NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos
NZY M-MuLV First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA.
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Dry Ice
NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos, is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. Random hexamers and Oligo(dT)18 primers are provided in separate tubes to offer the convenience to choose the appropriate primer to initiate your reverse-transcription reaction. The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY M-MuLV First-Strand cDNA Synthesis Kit, separate oligos, is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. Besides random hexamers and Oligo(dT)18 primers, the kit includes NZYRT 2× Master Mix, no oligos, which contains dNTPs, MgCl2 and an optimized RT buffer; 10× Annealing Buffer and NZYM-MuLV RT Enzyme Mix. NZYM-MuLV RT Enzyme Mix includes both NZY M-MuLV Reverse Transcriptase (RNase H minus) and NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reactions since PCR primers will bind more easily to the cDNA.
Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Flexible primer selection, High-yield | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 37 °C | Protocol time | Approx. 90 min. |
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– NZYM-MuLV RT Enzyme Mix
– NZYRT 2x Master Mix, no oligos
– 10× Annealing Buffer
– Random hexamer mix (50 ng/µL)
– Oligo(dT)18 primer mix (50 µM)
– NZY RNase H (E. coli)
– DEPC-treated H2O
– NZYRT 2x Master Mix, no oligos
– 10× Annealing Buffer
– Random hexamer mix (50 ng/µL)
– Oligo(dT)18 primer mix (50 µM)
– NZY RNase H (E. coli)
– DEPC-treated H2O
Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Flexible primer selection, High-yield | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 37 °C | Protocol time | Approx. 90 min. |
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Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Flexible primer selection, High-yield | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 37 °C | Protocol time | Approx. 90 min. |
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