Lyo NZYSupreme One-step RT-qPCR Probe Master Mix (2x)

Lyo NZYSupreme One-step RT-qPCR Probe Master Mix is a freezedried reaction mixture developed for first-strand cDNA synthesis and subsequent real-time PCR in a single tube. This offers great convenience and minimize the risk of errors and contaminations. The master mix, formulated as a 2× reaction mixture, is provided with an optimized reconstitution buffer designed to confer maximal levels of stability and enzymatic activity. After reconstitution, the mixture contains all components necessary for both cDNA synthesis and real-time PCR (including enzymes, dNTPs, stabilizers and enhancers), except primers, probes and RNA template. This master mix was engineered with a dual hotstart enzyme control mechanism to provide the highest detection sensitivity. In addition, the latest developments in PCR enhancers have been incorporated in the Lyo NZYSupreme One-step RT-qPCR Probe Master Mix, including buffer chemistry and incorporation of highly robust engineered enzymes. This master mix does not contain ROX and it was specifically developed for probe-detection technology. For qPCR instruments that require this reference dye, we indicate a concentration range for your assay according to the table shown in the section “ROX reference dye” (ROX not provided)