Description: YeOgl22A (PL22-PL22), E.C. number 188.8.131.52, is an enzyme that uses a β-elimination mechanism to catalyze the conversion of saturated and unsaturated digalacturonate into monogalacturonate and 4,5-unsaturated monogalacturonate-like molecules from Yersinia enterocolitica. Recombinant YeOgl22A (PL22-PL22), purified from Escherichia coli, is a modular family 22 Pectate Lyase (PL22) with two PL22 catalytic module repeats(www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: – 20 °C
Temp: 37 °C
Substrates: saturated and unsaturated digalacturonate (GalA2)