Endonuclease IV (Tth) (MB43101)

Refrigerated package

69,00

500 U

69,00

Get Any 4, Pay 3 - October 2021
We offer the cheapest product. Not valid for services.

Description: Endonuclease IV (Tth) is a Class II thermostable Apurinic/aPyrimidinic (AP) DNA endonuclease from Thermus thermophilus. This an enzyme is involved in the DNA Base Excision Repair (BER) pathway, that catalyses the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis, leaving a 1 nucleotide gap with 3′-hydroxyl and 5′ deoxyribose phosphate (dRP) termini. AP sites are locations in DNA that neither contain a purine nor a pyrimidine base, which occur either spontaneously or due to DNA damage. Endonuclease IV (Tth) displays activity in both ssDNA and dsDNA. The enzyme also displays 3’-diesterase activity.

Features:
– Thermostable DNA endonuclease
– Catalyses the cleavage of DNA phosphodiester backbone at AP sites generating a hydroxyl group at 3′-end and 5′ deoxyribose phosphate (dRP) terminus
– Displays activity in single-stranded DNA and double-stranded DNA

Applications:
– Studies of DNA damage
– Single cell electrophoresis (comet assay)
– Alkaline unwinding
– SNP analysis
– Genotyping

Specifications:

– Enzyme concentration: 10 U/μL
– Source: Recombinant E. coli strain
– Optimal reaction temperature: 65 °C
– Heat inactivation: no
– Storage conditions: Store at -20 °C
– Shipping conditions: Shipped at dry ice

Components:
– Endonuclease IV (Tth) (10 U/μL)
– Reaction Buffer (10x)

Product Brochure EN
Certificate of Analysis ZN041

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