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NZY M-MuLV First-Strand cDNA Synthesis Kit
NZY M-MuLV First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. The kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR.
NZY M-MuLV First-Strand cDNA Synthesis Kit is a system that includes all the necessary components to synthesize first-strand cDNA, except the template RNA. The resulting single-stranded cDNA is suitable for use in real-time quantitative Reverse Transcription PCR (RT-qPCR). NZY M-MuLV First-Strand cDNA Synthesis Kit is formulated to provide high yields of full-length cDNA products and to increase sensitivity in RT-qPCR. Starting material can range from 10 pg up to 5 µg of total RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYM-MuLV RT Enzyme Mix includes both NZY M-MuLV Reverse Transcriptase (RNase H minus) and NZY Ribonuclease Inhibitor in order to protect RNA against degradation due to ribonuclease contamination. RNase H (from E. coli) is provided in a separate tube to specifically degrade the RNA template in cDNA-RNA hybrids after the first-strand cDNA synthesis. This procedure will improve the sensitivity of subsequent RT-qPCR reaction since PCR primers will bind more easily to the cDNA.
Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Dual-primer: oligo(dT) + random hexamers, High yield | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 37 °C | Protocol time | Approx. 90 min. |
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– NZYM-MuLV RT Enzyme Mix
– NZYRT 2x Master Mix
– NZY RNase H (E. coli)
– DEPC-treated H2O
– NZYRT 2x Master Mix
– NZY RNase H (E. coli)
– DEPC-treated H2O
Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Dual-primer: oligo(dT) + random hexamers, High yield | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 37 °C | Protocol time | Approx. 90 min. |
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Shipping Conditions | Dry Ice | Storage Conditions | -85 °C to -15 °C | Application | cDNA synthesis, RT-PCR, RT-qPCR | Features | Dual-primer: oligo(dT) + random hexamers, High yield | Format | Kit, Liquid | Product Category | cDNA Synthesis | Reaction Temperature | 37 °C | Protocol time | Approx. 90 min. |
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CoA
Certificate of Analysis