Nt.BbvCI, Nicking Endonuclease (MB094)

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Description: Nt.BbvCI is a Nicking Endonuclease purified from an Escherichia coli strain that carries the BbvCI fragment from Brevibacillus brevis (Bacillus brevis). The enzyme cleaves only one strand of DNA on a double-stranded DNA substrate. Nt.BbvCI activity is blocked by some combinations of overlapping CpG methylation. NZYTech provides Nt.BbvCI with a specific reaction buffer. NZYTech’s Nt.BbvCI Nicking Endonuclease is an enzyme that acts at 37 °C.

Concentration: 10000 U/mL.

Unit definition: One unit is defined as the amount of this enzyme required to digest completely 1 μg of plasmid DNA in 50 μL of the reaction mixture at 37 °C for one hour.

Incubation temperature: 37 °C for one hour.

Heat inactivation: 80 °C for 20 minutes.

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