NZY Microbial gDNA Isolation kit (MB21702)


50 columns


Description: NZY Microbial gDNA Isolation kits are designed for the simple and rapid small-scale preparation of highly pure genomic DNA from a wide variety of microbial samples. This kit ensures cell wall lysis using mechanical disruption instead of enzymatic methods. Difficult to lyse microbial samples such as yeast, gram-positive bacteria and spores can be lysed using NZY Microbial gDNA Isolation kit. NZYSpin Microbial Bead Tubes replace enzymatic lysis and by mechanical disruption they can lyse strong complex cell wall structures. Bacterium and yeast such as Corynebacterium glutamicum, Saccharomyces cerevisiae, Bacillus subtilis and Escherichia coli are examples of microbial organisms whose DNA can be isolated using this kit. NZY Microbial gDNA Isolation kit is optimized to isolate 5-25 µg of DNA from up to 30 mg wet weight of microbial pellet, depending of the type of sample.

– High DNA yield and quality obtained from a range of samples
– Efficient sample lysis provided by ceramic beads (included in NZYSpin Microbial Bead Tubes)
– Silica-membrane technology
– Proteinase K (liquid) included

The purified genomic DNA is suitable for use in the most demanding molecular biology applications, including:
– Real-time quantitative PCR (qPCR)
– Genotyping
– DNA sequencing
– Southern blotting
– Enzimatic manipulations


Sample material (general): cell pellets of gram-positive and gram-negative bacteria, yeasts
Format: Spin-column
Sample amount: < 40 mg wet weight cell pellet
Typical yield: 5-25 µg (from 30 mg wet weight cell pellet)
A260/A280: 1.6-2.0
Elution volume: 100 µL
Binding capacity: 60 µg
Preparation time approx. 15 min/preparation
High-throughput compatibility: No
Product size: 50 columns
Storage conditions: Store all components at room temperature
Shipping conditions: Shipped at room temperature

– Buffer NML
– Buffer NMW1
– Buffer NMW2 (concentrate)
– Buffer NME
– Proteinase K (liquid)
– NZYSpin Microbial Bead Tubes
– NZYSpin Microbial Columns (light green rings)
– Collection tubes (2 mL)

Product Brochure EN
Certificate of Analysis YO111
Certificate of Analysis YL121
Certificate of Analysis 18061


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