Speedy NZYTaq 2x Green Master Mix (MB362)

65,00535,00

Description: Speedy NZYTaq 2× Green Master Mix is a premixed ready-to-use solution containing Speedy NZYTaq DNA polymerase (MB403), a recombinant DNA polymerase displaying a faster polymerization reaction than any other conventional non-proofreading enzyme. Only 5 seconds are required for the successful synthesis of 1 kb size DNA. The enzyme retains its speed (5 sec/kb) when amplifying fragments up to around 2-3 kb. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations for the efficient amplification of a wide range of DNA templates. MgCl2 final concentration is 2.5 mM, allowing the implementation of a variety of PCR protocols. In addition, reactions assembled with Speedy NZYTaq 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis.

Resulting PCR products have an A-overhang and are suitable for cloning with NZYTech´s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).

Features:
– Convenient ready-to-use PCR master mix
– Direct Gel Loading
– Fast routine amplification (5 sec/kb for DNA fragments up to 2-3 kb)
– High yields
– Leaves an A-overhang

Applications:
– Fast PCR
– Powerful PCR
– Colony PCR
– Generation of products for TA cloning

Specifications:

Product length: 0-6 kb
Hot-start-like capacity: no
Extension time: 5 sec/kb (or 10 sec/kb for targets higher than 2-3 kb in size) at 72 °C
3´→5´ activity: no
Product overhang: 3’-A
Storage conditions: Store at -20 ºC
Shipping conditions: Shipped at room temperature to dry ice

Components:
Speedy NZYTaq 2× Green Master Mix (0.2 U/μL)

Product Brochure EN
Safety Information EN
Informacao de Seguranca PT
Certificate of Analysis YO071
Certificate of Analysis YL101

1. What to do when there is no product amplification or low yield?
This can be due to the following situations:
a) Inadequate annealing temperature. The reaction mix composition may affect the melting properties of primers and DNA. Adjust the annealing temperature to accommodate the primer with the lowest melting temperature (5 °C to 10 °C lower than Tm).
b) Presence of PCR inhibitors. Some DNA isolation procedures, particularly genomic DNA isolation, can result in the co-purification of PCR inhibitors. Reduce the volume of template DNA in reaction or dilute template DNA prior to adding to the reaction. Diluting samples even 1:10,000 has been shown to be effective in improving results, depending on initial DNA concentration.
c) Concentration of magnesium is too low. Mg2+ is included in the Master Mix at a final concentration of 2.5 mM, which is sufficient for most targets. For some targets, higher Mg2+ concentration may be required. Titrate from 2.5 mM to 4 mM (final concentration) in 0.5 mM increments. (Note: MgCl2 is not provided in separate tubes).

2. How can I reduce the number of non-specific bands?
Adjust annealing conditions and/or design another set of primers, by increasing the length and avoiding complementary sequences.

3. How should I store my Speedy NZYTaq 2× Green Master Mix?
The product should be stored at -20 ºC, in a constant temperature freezer.

You may also like…

NZYDNA Ladder III

( MB044 ) 69,00139,00