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SKU
GF001

Gene Fragments

Storage Conditions:
-30 °C to -15 °C
NZYtech offers a fast and cost-effective Gene Fragments service providing double-stranded DNA sequences between 300-1750 bp in length. Gene Fragments constitute a simple option to PCR-based procedures and can be further used in every downstream cloning methods.
Availability
Produced On Demand
Unit Size
300-1750 bp
€0.00
NZYtech offers a fast and cost-effective Gene Fragments service providing double-stranded DNA sequences between 300-1750 bp in length. Gene Fragments constitute a simple option to PCR-based procedures and can be further used in every downstream cloning methods.
Gene Fragments sequences can be optimized using NZYtech’s proprietary gene optimization algorithm – ATGenium – which comprehensively optimizes critical factors in transcription, translation and co-translational protein folding to deliver highest levels of expression in any host system.

Features:
– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– Sequences length between 300 bp and 1750 bp
– Gene fragments size verification by electrophoresis
– Considerable saving of time and money
– Complete confidentiality guaranteed

Delivery Specifications:
– 400 ng dry dsDNA fragment
– Gene Fragments Report
More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Uncloned product delivery
Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech's proprietary codon optimization software – ATGenium - can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech's ATGenium algorithm.

ATGenium Expression levels
NZYCodon Optimiztion Algorithm
A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm
More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Uncloned product delivery
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More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Uncloned product delivery
More Information
Shipping Conditions Room Temperature to Dry Ice
Storage Conditions -30 °C to -15 °C
Features Uncloned product delivery

MSDS

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CoA

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