Site-directed Mutagenesis

Storage Conditions:

-30 °C to -15 °C

NZYtech offers a site-directed mutagenesis service which includes the introduction of single or multiple mutations – substitutions, insertions or deletions – into existing plasmid DNA sequences.

Availability

Produced On Demand

Details

NZYtech offers a site-directed mutagenesis service which includes the introduction of single or multiple mutations – substitutions, insertions or deletions – into existing plasmid DNA sequences. The strategy usually involves an oligonucleotide-directed mutagenic approach. All generated mutant clones are screened by Sanger sequencing to ensure that the desired modifications have been made.

Features:
– Substitution, deletion or insertion within a known plasmid DNA sequence
– Use of high-fidelity enzymes to minimize PCR errors
– 100% accuracy of the mutated region
– Short turnaround time
– Complete confidentiality guaranteed

Delivery Specifications:
– 40-4000 ng purified mutant plasmid
– Site-directed mutagenesis report containing QC certificate