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SKU
HS006

High-Throughput Gene Synthesis

Storage Conditions:
-85 °C to -15 °C
For orders above 24 synthetic genes, NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes.
Availability
Produced On Demand
Unit Size
2x8 Reactions
€0.00
NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes. With that in mind, we have improved standard molecular biology processes and developed innovative bioinformatics tools for gene design and codon optimization. HTP Gene Synthesis will allow you to tackle difficult research projects such as those involving determining structure-function relationships in different proteins, creating novel antibody libraries, screening gene variant libraries or optimizing protein function and expression. Our codon optimization technology – ATGenium – will allow obtaining the highest possible levels of gene expression in different expressions hosts while retaining high levels of protein solubility.
Synthetic genes can be cloned into our standard cloning vectors (pUC57), into pHTP Escherichia coli expression vectors or into a selection of other bacterial, mammalian, yeast and baculovirus/insect expression vectors. Check the complete list of these additional vectors below in "Additional Expression Vectors List" in the "Manuals" section.

Features:
– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– 100% sequence accuracy guaranteed
– Cloning vector (pUC57) with Ampicillin or Kanamycin resistance
– Possibility of cloning your synthetic gene directly into a selection of bacterial, mammalian, yeast and baculovirus/insect expression vectors, namely into pHTP E. coli expression vectors (for an additional price).
– Complete confidentiality guaranteed

Delivery Specifications:
– 40-4000 ng of purified plasmid
– Gene Synthesis Report
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech's proprietary codon optimization software – ATGenium - can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech's ATGenium algorithm.

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
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More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C
More Information
Shipping Conditions Dry Ice
Storage Conditions -85 °C to -15 °C

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