We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
SKU
MB00801
T4 Polynucleotide Kinase (T4 PNK)
T4 Polynucleotide Kinase catalyses the transfer of the terminal phosphate of ATP to 5′-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3′-mononucleotides.
Availability
Ready to Ship
Dry Ice
T4 Polynucleotide Kinase (T4 PNK) catalyses the transfer of the terminal phosphate of ATP to 5'-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3'-mononucleotides. This enzyme also possesses a 3'-phosphatase and 2', 3' cyclic phosphodiesterase activity. The enzyme can be used for the following applications: 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation; end-labelling DNA or RNA for use as probes, for DNA sequencing or for DNA-protein foot printing; removal of 3' phosphoryl groups.
Applications:
– End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
– 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
– Removal of 3´-phosphoryl groups
Applications:
– End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
– 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
– Removal of 3´-phosphoryl groups
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Activity | 3'-dephosphorylation, 5´-phosphorylation |
Product Category | Other Modifying Enzymes |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 500 U |
-T4 Polynucleotide Kinase (500 U)
-Reaction buffer (10x)
-Dilution buffer
-Reaction buffer (10x)
-Dilution buffer
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Activity | 3'-dephosphorylation, 5´-phosphorylation |
Product Category | Other Modifying Enzymes |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 500 U |
Shipping Conditions | Dry Ice |
---|---|
Storage Conditions | -85°C to -15°C |
Activity | 3'-dephosphorylation, 5´-phosphorylation |
Product Category | Other Modifying Enzymes |
Reaction Temperature | 37 °C |
Heat Inactivation | 65 °C |
Concentration | 500 U |
CoA
Certificate of Analysis