T4 Polynucleotide Kinase (T4 PNK)

T4 Polynucleotide Kinase (T4 PNK) catalyses the transfer of the terminal phosphate of ATP to 5'-hydroxyl termini of polynucleotides such as DNA and RNA, oligonucleotides and 3'-mononucleotides. This enzyme also possesses a 3'-phosphatase and 2', 3' cyclic phosphodiesterase activity. The enzyme can be used for the following applications: 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation; end-labelling DNA or RNA for use as probes, for DNA sequencing or for DNA-protein foot printing; removal of 3' phosphoryl groups.
Applications:
– End-labeling DNA or RNA for use as probes, for sequencing or for DNA-protein footprinting
– 5´-phosphorylation of oligonucleotides, PCR products, other DNA or RNA to allow subsequent ligation
– Removal of 3´-phosphoryl groups