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NZYMidiprep
NZMidiprep kit allows for rapid preparation of medium-scale highly pure plasmid DNA from E. coli strains.
NZYMidiprep kit is designed for the rapid, medium-scale preparation of highly pure plasmid DNA (typically 100 µg) from recombinant Escherichia coli strains. Plasmid DNA binds selectively to NZYtech columns charged with a silica-based anion-exchange resin.
All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 100 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.
All contaminants, such as proteins, RNA, salts, nucleotides and oligos (<40-mer) are washed from the column. In the elution step, the positive charge of the resin is neutralized by a pH shift to slightly alkaline conditions and pure plasmid DNA is eluted in a high-salt elution buffer. To isolate DNA from low copy number plasmids, BACs or cosmids, or to obtain higher DNA concentrations, use 100 mL of E. coli cultures and double the volumes of Buffers M1, M2 and M3.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Midiprep | Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 90 min. |
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– Buffer M1
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Midi Columns
– Buffer M2
– Buffer M3
– Buffer MEQ
– Buffer MW
– Buffer ME
– RNase A
– NZYTech Plasmid Midi Columns
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Midiprep | Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 90 min. |
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Using a Dual CRISPR/Cas9 Approach to Gain Insight into the Role of LRP1B in Glioblastoma.
Peixoto J, Príncipe C, Pestana A, Osório H, Teixeira Pinto M, Prazeres H, Soares P, Lima R
International Journal of Molecular Sciences (IJMS), 2023.
Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells.
Pereira I, da Cunha M, Pacheco Leal I, Pequito Luís M, Gonçalves P, Gonçalves C, Jaime Mota L.
PLOS Pathogens, 2024.
Condensation and Protection of DNA by the Myxococcus xanthus Encapsulin: A Novel Function.
Almeida A, Carvalho A, Calmeiro T, Jones N, Hoffmann S, Fortunato E, Pereira A, Tavares P.
International Journal of Molecular Sciences, 2022.
The Chlamydia trachomatis inclusion membrane protein CT006 associates with lipid droplets in eukaryotic cells.
Bugalhão J, Luís M, Pereira I, da Cunha M, Pais S, Jaime Mota L.
PLOS ONE, 2022.
Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells.
Serrano Pereira I, da Cunha M, Pacheco Leal I, Pequito Luís M, Gonçalves P, Gonçalves C, Jaime Mota L.
Medical Microbiology and Immunology, 2024.
The Chlamydia trachomatis type III secretion substrates CT142, CT143, and CT144 are secreted into the lumen of the inclusion.
da Cunha M, Pais SV, Bugalhão JN, Mota LJ.
PLoS One, 2017.
Structure-based virtual screening toward the discovery of novel inhibitors of the DNA repair activity of the human apurinic/apyrimidinic endonuclease 1.
Guerreiro PS, Estácio SG, Antunes F, Fernandes AS, Pinheiro PF, Costa JG, Castro M, Miranda JP, Guedes RC, Oliveira NG.
Chem Biol Drug Des, 2016.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Midiprep | Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 90 min. |
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Isolation of high-copy number plasmid DNA (in duplicate) using NZYMidiprep (1) or a competitor plasmid purification kit (2). Lane M: NZYDNA Ladder III.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Midiprep | Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 90 min. |
|---|
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Midiprep | Features | Manual, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 90 min. |
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SDS
Safety Data Sheets
CoA
Certificate of Analysis
