Description: SpAcp18B (GH18), E.C. number 18.104.22.168, is an enzyme that participates in the endohydrolysis of the diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact from Streptomyces plicatus. Recombinant SpAcp18B (GH18), purified from Escherichia coli, is a single domain family 18 Glycoside Hydrolase (GH18) (www.cazy.org). The enzyme is provided in 35 mM NaHepes buffer, pH 7.5, 750 mM NaCl, 200 mM imidazol, 3.5 mM CaCl2, 0.02% sodium azide and 25% (v/v) glycerol, at a 1 mg/mL concentration. Bulk quantities of this product are available on request.
Storage: – 20 °C
Temp: 37 °C
Substrates: N-linked glycans in glycoproteins