phi29 DNA Polymerase (MB42301)

Refrigerated package

49,00

250 U

49,00

Description: phi29 DNA Polymerase is a highly processive non-thermostable polymerase displaying strong strand displacement activity, which allows highly efficient isothermal DNA amplification. phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 and possesses a strong 3’→5′ exonuclease (proofreading) activity. It acts preferentially on single-stranded DNA or RNA. phi29 DNA Polymerase is particular efficient for Whole Genome Amplification (WGA) through Multiple Displacement Amplification (MDA).

Features:
– Highly processive DNA polymerase
– Powerful strand displacement activity
– High fidelity – strong 3’→5′ exonuclease (proofreading) activity
– Suitable for isothermal amplification – no need for a thermocycler

Applications:
– Isothermal amplification
– Whole Genome Amplification (WGA)
– Multiple displacement amplification (MDA)
– DNA template preparation for sequencing

Specifications:

Enzyme concentration:10 U/μL
Source:Recombinant E. coli strain
Optimal reaction temperature:30 °C
Heat inactivation:65 °C for 10 min
Storage conditions:Store at -20 °C
Shipping conditions:Shipped at dry ice

Components:
– phi29 DNA Polymerase (10 U/μL)
– Reaction Buffer (10x)

Product Brochure EN
Certificate of Analysis ZN021

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