phi29 DNA Polymerase (MB42301)

Refrigerated package


250 U


Description: phi29 DNA Polymerase is a highly processive non-thermostable polymerase displaying strong strand displacement activity, which allows highly efficient isothermal DNA amplification. phi29 DNA Polymerase is the replicative polymerase from the Bacillus subtilis phage phi29 and possesses a strong 3’→5′ exonuclease (proofreading) activity. It acts preferentially on single-stranded DNA or RNA. phi29 DNA Polymerase is particular efficient for Whole Genome Amplification (WGA) through Multiple Displacement Amplification (MDA).

– Highly processive DNA polymerase
– Powerful strand displacement activity
– High fidelity – strong 3’→5′ exonuclease (proofreading) activity
– Suitable for isothermal amplification – no need for a thermocycler

– Isothermal amplification
– Whole Genome Amplification (WGA)
– Multiple displacement amplification (MDA)
– DNA template preparation for sequencing


Enzyme concentration: 10 U/μL
Source: Recombinant E. coli strain
Optimal reaction temperature: 30 °C
Heat inactivation: 65 °C for 10 min
Storage conditions: Store at -20 °C
Shipping conditions: Shipped at dry ice

– phi29 DNA Polymerase (10 U/μL)
– Reaction Buffer (10x)

Product Brochure EN
Certificate of Analysis ZN021

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