Benchmark Analysis: NZYSupreme Multiplex One-Step RT-qPCR Probe Master Mix

Enhanced Detection and Robust Amplification

NZYSupreme Multiplex One-Step RT-qPCR Probe Master Mix is a high performing master mix, combining sensitivity and eciency for fast and accurate results. Benchmarked against a total of 4 market-leading master mixes considered to be the gold-standard in qPCR Master Mixes, the MB442 proved to be a leading-edge product with first-class results.

Amplification of SARS-CoV-2 genes in different contexts

A 10-fold serial dilution of RNA carrying the SARS-CoV-2 genes RdRp (for virus RNA dependent RNA polymerase) and
N (for Nucleocapsid phosphoprotein) was used as template to detect SARS-CoV-2 in three dierent real-time qPCR experiments:

• The reaction mixture contains primers and probes to amplify SARS-CoV-2 genes (RdRp and N), in the presence of SARS-CoV-2 RNA.
• The reaction mixture contains, in addition to SARS-CoV-2 primers and probes to amplify RdRp and N genes, primers and probes to simultaneously detect Influenza A (INFA) and Influenza B (INFB), as well as human RNase P, although only SARS-CoV-2 RNA is present.
• The reaction mixture contains, in addition to SARS-CoV-2 primers and probes to amplify RdRp and N genes, primers and probes to simultaneously detect Influenza A (INFA) and Influenza B (INFB), as well as human RNase P, in the context of a co-infection scenario where nucleic acids of all dierent species are present. Probes were dierently labeled to allow simultaneously detection of the three species –SARS-CoV-2 (JUN); Influenzavirus A and B (FAM); human RNase P (VIC).

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Benchmark Analysis - NZYSupreme Multiplex One-Step RT-qPCR Probe Master Mix